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Anti-Human WDR5 IP Recombinant Antibody (SAA0239) (HX013013)

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  • 50ug
  • 100ug
  • 1mg
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概述
货号HX013013
品牌abinScience
种属反应性Human
应用ELISA, FCM, IF, IP
同种型IgG1
克隆号SAA0239
靶标WDR5, BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, BIG3
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P61964
RRIDAnti-Human WDR5 IP Antibody (SAA0239) (abinScience Cat# HX013013, RRID:AB_3729773)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

WD repeat-containing protein 5 (WDR5) is a ~36 kDa protein. Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.

1. Han, Z. et al. (2006) Molecular cell 22, 137-44. PMID: 16600877
2. Couture, JF. et al. (2006) Nature structural & molecular biology 13, 698-703. PMID: 16829960
3. Guelman, S. et al. (2009) Molecular and cellular biology 29, 1176-88. PMID: 19103755
4. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338
5. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245
6. Cai, Y. et al. (2010) The Journal of biological chemistry 285, 4268-72. PMID: 20018852
7. Song, JJ. et al. (2008) The Journal of biological chemistry 283, 35258-64. PMID: 18840606
8. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120
9. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653
NoteFor research use only.
Isotype ControlMouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
图片
  • Flow Cytometry

    Flow-cytometry using anti-human WDR5 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human WDR5 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human WDR5 IP Antibody (SAA0239).

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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