Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Cyclin-dependent kinase 9, 2.7.11.22, 2.7.11.23, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit, CDK9, CDC2L4, TAK
背景
Cyclin-dependent kinase 9 (CDK9) is a ~42 kDa protein. Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELFE. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g.
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2. Ivanov, D. et al. (2000) Molecular and cellular biology 20, 2970-83. PMID: 10757782
3. Kim, JB. et al. (2001) The Journal of biological chemistry 276, 12317-23. PMID: 11145967
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5. Bourgeois, CF. et al. (2002) Molecular and cellular biology 22, 1079-93. PMID: 11809800
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7. Fujinaga, K. et al. (2004) Molecular and cellular biology 24, 787-95. PMID: 14701750
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10. Yu, DS. et al. (2010) EMBO reports 11, 876-82. PMID: 20930849
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-CDK9 polyclonal antibody at 1ug/mL on various samples. Lane 1: Mouse lung lysate Lane 2: Rat brain lysate Lane 3: Rat lung lysate
