Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes.
1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748
2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464
3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535
4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063
5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875
6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204
