Anti-ERN1 Polyclonal Antibody (HF537014)

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概述
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产品说明书

概述

货号

HF537014

品牌

abinScience

描述

Anti-ERN1 Polyclonal Antibody (HF537014) is a rabbit polyclonal antibody detecting ERN1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ERN1 (Phe571-Leu977).

靶标

Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, ERN1, Ire1-alpha, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Inositol-requiring protein 1, IRE1

纯化方式

Purified by antigen affinity column.

Accession号

O75460

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes.

1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748

2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464

3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535

4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063

5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875

6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204

7. Chang, TK. et al. (2018) Molecular cell 71, 629-636.e5. PMID: 30118681

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Tirasophon, W. et al. (1998) Genes & development 12, 1812-24. PMID: 9637683

10. Eletto, D. et al. (2014) Molecular cell 53, 562-576. PMID: 24508390

Note

For research use only.

图片

参考文献

产品说明书

Anti-ERN1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ERN1 Polyclonal Antibody (HF537014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ERN1 Polyclonal Antibody (HF537014)

概述

图片

参考文献

产品说明书

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