Affinity Purified — Minimal background and high purity for reliable results.
Multi-Application — Validated across multiple applications.
Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human SQSTM1 (Met1-Val122).
靶标
OSIL,EBIAP,Sequestosome-1,Ubiquitin-binding protein p62,ORCA,EBI3-associated protein of 60 kDa,p60,SQSTM1,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Sequestosome-1 (SQSTM1/p62) is a ~47 kDa protein. Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes. Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps. SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies. SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins. SQSTM1 is itself degraded along with its ubiquitinated cargos.
1. Seibenhener, ML. et al. (2004) Molecular and cellular biology 24, 8055-68. PMID: 15340068
2. Babu, JR. et al. (2005) Journal of neurochemistry 94, 192-203. PMID: 15953362
3. Bjørkøy, G. et al. (2005) The Journal of cell biology 171, 603-14. PMID: 16286508
4. Pankiv, S. et al. (2007) The Journal of biological chemistry 282, 24131-45. PMID: 17580304
