Recombinant Human SQSTM1 Protein, N-His (PX-P5936)
概述
图片
参考文献
概述
货号
PX-P5936
品牌
ProteoGenix
描述
Recombinant Human SQSTM1 Protein, N-His (PX-P5936) expressed in E. coli, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
E. coli Expression — Optimized for functional studies.
表达系统
E. coli
Accession号
Q13501
蛋白长度
Met1-Val122
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
15.99 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
OSIL, EBIAP, Sequestosome-1, Ubiquitin-binding protein p62, ORCA, EBI3-associated protein of 60 kDa, p60, SQSTM1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
背景
Sequestosome-1 (SQSTM1/p62) is a ~47 kDa protein. Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes. Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps. SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies. SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins. SQSTM1 is itself degraded along with its ubiquitinated cargos.
1. Seibenhener, ML. et al. (2004) Molecular and cellular biology 24, 8055-68. PMID: 15340068
2. Babu, JR. et al. (2005) Journal of neurochemistry 94, 192-203. PMID: 15953362
3. Bjørkøy, G. et al. (2005) The Journal of cell biology 171, 603-14. PMID: 16286508
4. Pankiv, S. et al. (2007) The Journal of biological chemistry 282, 24131-45. PMID: 17580304
