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Anti-RABV Glycoprotein G Polyclonal Antibody, Biotin (VK632324)

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概述
货号VK632324
品牌abinScience
描述
Anti-Rabies virus/RABV G/Glycoprotein Polyclonal Antibody, Biotin (VK632324) is a rabbit polyclonal antibody detecting Glycoprotein in WB, IHC, ELISA.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Biotin Conjugated — Signal amplification with streptavidin systems.
种属反应性Rabies virus (RABV)
应用WB, IHC, ELISA
宿主Rabbit
克隆类型Polyclonal
同种型IgG
偶联物Biotin
免疫原E. coli - derived recombinant Rabies virus/RABV G/Glycoprotein (Leu30-Tyr459).
靶标G, Glycoprotein
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
纯化方式Purified by antigen affinity column.
Accession号O92284
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Glycoprotein is a ~58 kDa protein. Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells.

NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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