Anti-Human B7-2/CD86 Recombinant Antibody (3D1) (HW776023)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HW776023

abinScience

种属反应性

Human

ELISA, FCM, IP

IgG1, kappa

经测试应用

WB, FCM

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2, B7-2

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P42081, K7GMU1

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

T-lymphocyte activation antigen CD86 (B7-2) is a ~37 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.

1. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

2. Freeman, GJ. et al. (1993) Science (New York, N.Y.) 262, 909-11. PMID: 7694363

3. Lanier, LL. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 97-105. PMID: 7527824

4. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501

For research use only

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Western blot

Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD86/B7-2 (Catalog No: HW776011)
Lane 2: negative control
Flow cytometry

Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 monoclonal antibody (Catalog HW776023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human B7-2/CD86 Recombinant Antibody (3D1).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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Anti-Human B7-2/CD86 Recombinant Antibody (3D1) (HW776023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human B7-2/CD86 Recombinant Antibody (3D1) (HW776023)

概述

图片

参考文献

产品说明书

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