Anti-Human NMI Polyclonal Antibody (HD442014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HD442014

品牌

abinScience

描述

Anti-Human NMI Polyclonal Antibody (HD442014) is a rabbit polyclonal antibody detecting NMI in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human NMI (Phe58-Glu307).

靶标

N-myc and STAT interactor, N-myc-interactor, NMI, Nmi

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q13287

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

Q13287

背景

N-myc-interactor (NMI) is a ~35 kDa protein. Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21.

1. Das, A. et al. (2015) Virology 485, 383-92. PMID: 26342464

2. Xiahou, Z. et al. (2017) Nature communications 8, 950. PMID: 29038465

3. Jian, D. et al. (2018) Acta physiologica (Oxford, England) 223, e13037. PMID: 29350881

4. Zhu, M. et al. (1999) Cell 96, 121-30. PMID: 9989503

5. Feng, L. et al. (2018) PLoS pathogens 14, e1006867. PMID: 29377960

6. Zhou, X. et al. (2000) The Journal of biological chemistry 275, 21364-71. PMID: 10779520

7. Chen, J. et al. (2000) The Journal of biological chemistry 275, 36278-84. PMID: 10950963

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-NMI polyclonal antibody at 1ug/mL on various samples.
Lane 1: PC-3 cell lysate

参考文献

产品说明书

Anti-Human NMI Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NMI Polyclonal Antibody (HD442014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NMI Polyclonal Antibody (HD442014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价