ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
30.64 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
N-myc and STAT interactor, N-myc-interactor, NMI, Nmi
背景
N-myc-interactor is a ~35 kDa protein. Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21.
1. Das, A. et al. (2015) Virology 485, 383-92. PMID: 26342464
2. Xiahou, Z. et al. (2017) Nature communications 8, 950. PMID: 29038465
3. Jian, D. et al. (2018) Acta physiologica (Oxford, England) 223, e13037. PMID: 29350881
4. Zhu, M. et al. (1999) Cell 96, 121-30. PMID: 9989503
5. Feng, L. et al. (2018) PLoS pathogens 14, e1006867. PMID: 29377960
6. Zhou, X. et al. (2000) The Journal of biological chemistry 275, 21364-71. PMID: 10779520
7. Chen, J. et al. (2000) The Journal of biological chemistry 275, 36278-84. PMID: 10950963
