



| 货号 | MY257013 | ||||
|---|---|---|---|---|---|
| 品牌 | abinScience | ||||
| 种属反应性 | Mouse | ||||
| 应用 | ELISA, FCM, Functional assay, Research in vivo | ||||
| 宿主 | Mouse | ||||
| 克隆类型 | Monoclonal | ||||
| 同种型 | IgG2c, kappa | ||||
| 克隆号 | MB20-11 | ||||
| 经测试应用 | FCM | ||||
| 靶标 | B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1 | ||||
| 内毒素水平 | < 10 EU/mg | ||||
| 纯度 | >95% as determined by SDS-PAGE. | ||||
| 纯化方式 | Protein A/G purified from cell culture supernatant. | ||||
| Accession号 | P19437 | ||||
| 状态 | Liquid | ||||
| 保存溶液 | 0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 背景 | B-lymphocyte antigen CD20 (MS4A1) is a ~31 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva). | ||||
| Note | For research use only |

Flow-cytometry using FITC anti-Mouse CD20 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC conjugated anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing,cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-Mouse CD20 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience:MF690414) and cells analysed on a NovoCyte Flow Cytometer.





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