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Anti-Escherichia coli eae/Intimin Polyclonal Antibody (ARO-A11919)

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概述
货号ARO-A11919
品牌ProteoGenix
描述
Anti-Escherichia coli eae/Intimin Polyclonal Antibody (ARO-A11919) is a rabbit polyclonal antibody detecting Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939 in ELISA, IHC, WB. Suitable for Escherichia coli O127:H6 (strain E2348/69 / EPEC).
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Escherichia coli O127:H6 (strain E2348/69 / EPEC)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Escherichia coli eae/Intimin (Lys450-Gln655).
靶标Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939
纯化方式Purified by antigen affinity column.
Accession号P19809
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Intimin (eae) is a ~102 kDa protein. An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells. Necessary for the production of attaching and effacing lesions on infected human tissue culture cells. Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable).

1. Leo, JC. et al. (2015) Molecular microbiology 95, 80-100. PMID: 25353290
2. Weikum, J. et al. (2020) Scientific reports 10, 21249. PMID: 33277518
3. Jerse, AE. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7839-43. PMID: 2172966
4. Batchelor, M. et al. (2000) The EMBO journal 19, 2452-64. PMID: 10835344
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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