Affinity Purified — Minimal background and high purity for reliable results.
Multi-Application — Validated across multiple applications.
种属反应性
Human
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human BHLHE40 (Ser46-Gly186).
靶标
DEC1, bHLHe40, Differentially expressed in chondrocytes protein 1, Class E basic helix-loop-helix protein 40, Class B basic helix-loop-helix protein 2, STRA13, SHARP2, SHARP-2, bHLHb2, Enhancer-of-split and hairy-related protein 2, BHLHB2, BHLHE40, Stimulated by retinoic acid gene 13 protein
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Class E basic helix-loop-helix protein 40 (BHLHE40) is a ~45 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.
1. Honma, S. et al. (2002) Nature 419, 841-4. PMID: 12397359
2. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297
3. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706
4. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144
5. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782
6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558
7. Marczak, MM. et al. (2017) Archives of biochemistry and biophysics 631, 11-18. PMID: 28797635
8. Nakashima, A. et al. (2018) Hypertension (Dallas, Tex. : 1979) 72, 746-754. PMID: 30012868
