ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
18.31 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
DEC1, bHLHe40, Differentially expressed in chondrocytes protein 1, Class E basic helix-loop-helix protein 40, Class B basic helix-loop-helix protein 2, STRA13, SHARP2, SHARP-2, bHLHb2, Enhancer-of-split and hairy-related protein 2, BHLHB2, BHLHE40, Stimulated by retinoic acid gene 13 protein
背景
Class E basic helix-loop-helix protein 40 (BHLHE40) is a ~45 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.
1. Honma, S. et al. (2002) Nature 419, 841-4. PMID: 12397359
2. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297
3. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706
4. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144
5. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782
6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558
7. Marczak, MM. et al. (2017) Archives of biochemistry and biophysics 631, 11-18. PMID: 28797635
8. Nakashima, A. et al. (2018) Hypertension (Dallas, Tex. : 1979) 72, 746-754. PMID: 30012868
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human BHLHE40 Protein, N-His
