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Anti-STAT4 Polyclonal Antibody (ARO-A13799)

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概述
货号ARO-A13799
品牌ProteoGenix
描述
Anti-STAT4 Polyclonal Antibody (ARO-A13799) is a rabbit polyclonal antibody detecting Signal transducer and activator of transcription 4, STAT4 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human STAT4 (Tyr505-Ile737).
靶标Signal transducer and activator of transcription 4, STAT4
纯化方式Purified by antigen affinity column.
Accession号Q14765
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Signal transducer and activator of transcription 4 (STAT4) is a ~85 kDa protein. Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes.

1. Visconti, R. et al. (2000) Blood 96, 1844-52. PMID: 10961885
2. Baghdassarian, H. et al. (2023) The New England journal of medicine 388, 2241-2252. PMID: 37256972
3. Cho, SS. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 4781-9. PMID: 8943379
4. Morinobu, A. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 12281-6. PMID: 12213961
5. Zhang, YS. et al. (2022) Cell death and differentiation 29, 2303-2315. PMID: 35614130
6. Yao, BB. et al. (1999) Archives of biochemistry and biophysics 368, 147-55. PMID: 10415122
7. Bacon, CM. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7307-11. PMID: 7638186
8. Oppmann, B. et al. (2000) Immunity 13, 715-25. PMID: 11114383
9. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746
10. Gonzales-van Horn, SR. et al. (2016) European journal of immunology 46, 1504-10. PMID: 26990433
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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