ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
28.84 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Signal transducer and activator of transcription 4, STAT4
背景
Signal transducer and activator of transcription 4 (STAT4) is a ~85 kDa protein. Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes.
1. Visconti, R. et al. (2000) Blood 96, 1844-52. PMID: 10961885
2. Baghdassarian, H. et al. (2023) The New England journal of medicine 388, 2241-2252. PMID: 37256972
3. Cho, SS. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 4781-9. PMID: 8943379
4. Morinobu, A. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 12281-6. PMID: 12213961
5. Zhang, YS. et al. (2022) Cell death and differentiation 29, 2303-2315. PMID: 35614130
6. Yao, BB. et al. (1999) Archives of biochemistry and biophysics 368, 147-55. PMID: 10415122
7. Bacon, CM. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7307-11. PMID: 7638186
8. Oppmann, B. et al. (2000) Immunity 13, 715-25. PMID: 11114383
9. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746
10. Gonzales-van Horn, SR. et al. (2016) European journal of immunology 46, 1504-10. PMID: 26990433
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human STAT4 Protein, N-His
