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Anti-OXCT1 Polyclonal Antibody (ARO-A13906)

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概述
货号ARO-A13906
品牌ProteoGenix
描述
Anti-OXCT1 Polyclonal Antibody (ARO-A13906) is a rabbit polyclonal antibody detecting 3-oxoacid CoA-transferase 1, OXCT1, Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, OXCT, SCOT, SCOT-s in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pig.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat, Pig
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human OXCT1 (Thr40-Leu489).
靶标3-oxoacid CoA-transferase 1, OXCT1, Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, OXCT, SCOT, SCOT-s
纯化方式Purified by antigen affinity column.
Accession号P55809
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (OXCT1) is a ~56 kDa protein. Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production. Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

1. Fukao, T. et al. (2000) Genomics 68, 144-51. PMID: 10964512
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Anti-OXCT1 Polyclonal Antibody

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