Anti-OXCT1 Polyclonal Antibody (HX904014)

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概述
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产品说明书

概述

货号

HX904014

品牌

abinScience

描述

Anti-OXCT1 Polyclonal Antibody (HX904014) is a rabbit polyclonal antibody detecting OXCT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human OXCT1 (Thr40-Leu489).

靶标

3-oxoacid CoA-transferase 1, OXCT1, Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, OXCT, SCOT, SCOT-s

纯化方式

Purified by antigen affinity column.

Accession号

P55809

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (OXCT1) is a ~56 kDa protein. Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production. Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

1. Fukao, T. et al. (2000) Genomics 68, 144-51. PMID: 10964512

Note

For research use only.

图片

Western blot

Lane 1: Mouse heart lysate
Lane 2: Mouse kidney lysate
Lane 3: Rat heart lysate

参考文献

产品说明书

Anti-OXCT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-OXCT1 Polyclonal Antibody (HX904014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-OXCT1 Polyclonal Antibody (HX904014)

概述

图片

参考文献

产品说明书

相关产品推荐

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