Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
DNA replication licensing factor MCM2 is a ~101 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425
2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004
3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328
4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232
5. Todorov, IT. et al. (1994) Journal of cell science 107 ( Pt 1), 253-65. PMID: 8175912
6. Gao, J. et al. (2015) PloS one 10, e0133522. PMID: 26196677
