Anti-MCM2 Polyclonal Antibody (HW401014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

HW401014

品牌

abinScience

描述

Anti-MCM2 Polyclonal Antibody (HW401014) is a rabbit polyclonal antibody detecting MCM2 in ELISA, IHC, WB. Suitable for Human, Mouse, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MCM2 (Phe657-Phe904).

靶标

CCNL1, KIAA0030, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, CDCL1, BM28, DNA replication licensing factor MCM2

纯化方式

Purified by antigen affinity column.

Accession号

P49736

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA replication licensing factor MCM2 is a ~101 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

5. Todorov, IT. et al. (1994) Journal of cell science 107 ( Pt 1), 253-65. PMID: 8175912

6. Gao, J. et al. (2015) PloS one 10, e0133522. PMID: 26196677

Note

For research use only.

图片

参考文献

产品说明书

Anti-MCM2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MCM2 Polyclonal Antibody (HW401014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MCM2 Polyclonal Antibody (HW401014)

概述

图片

参考文献

产品说明书

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