Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PCK1) is a ~69 kDa protein. Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis. Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis.
1. Adams, DR. et al. (2014) Molecular genetics and metabolism 113, 161-70. PMID: 24863970
2. Santra, S. et al. (2016) Molecular genetics and metabolism 118, 21-7. PMID: 26971250
3. Vieira, P. et al. (2017) Molecular genetics and metabolism 120, 337-341. PMID: 28216384
4. Latorre-Muro, P. et al. (2018) Molecular cell 71, 718-732.e9. PMID: 30193097
5. Xu, D. et al. (2020) Nature 580, 530-535. PMID: 32322062
