Anti-PCK1 Polyclonal Antibody (HW538014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HW538014

品牌

abinScience

描述

Anti-PCK1 Polyclonal Antibody (HW538014) is a rabbit polyclonal antibody detecting PCK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human PCK1 (Asp311-Pro559).

靶标

PCK1, PEPCK1, PEPCK-C, Serine-protein kinase PCK1, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

纯化方式

Purified by antigen affinity column.

Accession号

P35558

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PCK1) is a ~69 kDa protein. Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis. Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis.

1. Adams, DR. et al. (2014) Molecular genetics and metabolism 113, 161-70. PMID: 24863970

2. Santra, S. et al. (2016) Molecular genetics and metabolism 118, 21-7. PMID: 26971250

3. Vieira, P. et al. (2017) Molecular genetics and metabolism 120, 337-341. PMID: 28216384

4. Latorre-Muro, P. et al. (2018) Molecular cell 71, 718-732.e9. PMID: 30193097

5. Xu, D. et al. (2020) Nature 580, 530-535. PMID: 32322062

Note

For research use only.

图片

参考文献

产品说明书

Anti-PCK1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PCK1 Polyclonal Antibody (HW538014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PCK1 Polyclonal Antibody (HW538014)

概述

图片

参考文献

产品说明书

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