Anti-Clostridioides difficile Toxin B/tcdB Reference Antibody (Ombetoxabart, RUO) (JN876026)

价格:

规格：

100ug
1mg
5mg
10mg
25mg
50mg
100mg

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

JN876026

品牌

abinScience

描述

Research Grade ombetoxabart (JN876026) is a human monoclonal antibody. Suitable for Clostridioides difficile.

种属反应性

Clostridioides difficile

应用

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

宿主

Human

同种型

IgG1, kappa

克隆类型

Monoclonal

内毒素水平

< 10 EU/mg

纯度

>95% as determined by SDS-PAGE.

纯化方式

Protein A/G purified from cell culture supernatant.

Accession号

P18177

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149

3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933

5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463

6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

Note

For research use only.

图片

参考文献

产品说明书

Anti-Clostridioides difficile Toxin B/tcdB Reference Antibody (Ombetoxabart, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridioides difficile Toxin B/tcdB Reference Antibody (Ombetoxabart, RUO) (JN876026)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridioides difficile Toxin B/tcdB Reference Antibody (Ombetoxabart, RUO) (JN876026)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价