Anti-Human CDT1 Polyclonal Antibody (HD509014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HD509014

品牌

abinScience

描述

Anti-Human CDT1 Polyclonal Antibody (HD509014) is a rabbit polyclonal antibody detecting CDT1. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CDT1 (Thr112-Ser386).

靶标

CDT1, DNA replication factor Cdt1, DUP, Double parked homolog

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9H211

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

DNA replication factor Cdt1 is a ~60 kDa protein. Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene.

1. Wohlschlegel, JA. et al. (2000) Science (New York, N.Y.) 290, 2309-12. PMID: 11125146

2. Sugimoto, N. et al. (2004) The Journal of biological chemistry 279, 19691-7. PMID: 14993212

3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198

4. Varma, D. et al. (2012) Nature cell biology 14, 593-603. PMID: 22581055

5. Franz, A. et al. (2016) Nature communications 7, 10612. PMID: 26842564

6. Cook, JG. et al. (2004) The Journal of biological chemistry 279, 9625-33. PMID: 14672932

Note

For research use only

图片

Western blot

Lane 1: HCT116 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: Hela cell lysate

参考文献

产品说明书

Anti-Human CDT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CDT1 Polyclonal Antibody (HD509014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CDT1 Polyclonal Antibody (HD509014)

概述

图片

参考文献

产品说明书

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