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Human CDT1 Recombinant Protein (N-His) (HD509012)

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概述
货号HD509012
品牌abinScience
描述
Recombinant Human CDT1 Protein, N-His (HD509012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
表达系统E. coli
Accession号Q9H211
蛋白长度Thr112-Ser386
应用ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属Homo sapiens (Human)
性质Recombinant
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
状态Lyophilized
保存溶液 Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名CDT1, DNA replication factor Cdt1, DUP, Double parked homolog
背景

DNA replication factor Cdt1 is a ~60 kDa protein. Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene.

1. Wohlschlegel, JA. et al. (2000) Science (New York, N.Y.) 290, 2309-12. PMID: 11125146
2. Sugimoto, N. et al. (2004) The Journal of biological chemistry 279, 19691-7. PMID: 14993212
3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198
4. Varma, D. et al. (2012) Nature cell biology 14, 593-603. PMID: 22581055
5. Franz, A. et al. (2016) Nature communications 7, 10612. PMID: 26842564
6. Cook, JG. et al. (2004) The Journal of biological chemistry 279, 9625-33. PMID: 14672932
NoteFor research use only
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参考文献
产品说明书
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Human CDT1 Recombinant Protein (N-His)

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