Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
背景
Beta-secretase 2 (BACE2) is a ~56 kDa protein. Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Involved in the proteolytic shedding of PMEL at early stages of melanosome biogenesis. Cleaves PMEL within the M-beta fragment to release the amyloidogenic PMEL luminal fragment containing M-alpha and a small portion of M-beta N-terminus.
1. Yan, R. et al. (1999) Nature 402, 533-7. PMID: 10591213
2. Hussain, I. et al. (2000) Molecular and cellular neurosciences 16, 609-19. PMID: 11083922
3. Yan, R. et al. (2001) The Journal of biological chemistry 276, 34019-27. PMID: 11423558
4. Sun, X. et al. (2005) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19, 739-49. PMID: 15857888
5. Sun, X. et al. (2006) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20, 1369-76. PMID: 16816112
6. Rochin, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10658-63. PMID: 23754390
7. Esterházy, D. et al. (2011) Cell metabolism 14, 365-77. PMID: 21907142
