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Anti-Human DGKD Polyclonal Antibody (HV696014)

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概述
货号HV696014
品牌abinScience
描述
Anti-Human DGKD Polyclonal Antibody (HV696014) is a rabbit polyclonal antibody detecting Human DGKD. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human DGKD (Ser56-Asn314).
靶标DAG kinase eta, DGK-eta, DGKH, Diacylglycerol kinase eta, Diglyceride kinase eta, EC:2.7.1.107
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号Q86XP1
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
背景

Diacylglycerol kinase eta (DGKD) is a ~134 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

1. Murakami, T. et al. (2003) The Journal of biological chemistry 278, 34364-72. PMID: 12810723
2. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095
3. Yasuda, S. et al. (2009) The Journal of biological chemistry 284, 29559-70. PMID: 19710016
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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