Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HD297014

品牌

abinScience

描述

Anti-Human SPOUT1 Polyclonal Antibody (HD297014) is a rabbit polyclonal antibody detecting SPOUT1. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SPOUT1 (Thr74-Thr376).

靶标

28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q5T280

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

28S rRNA (uridine-N(3))-methyltransferase (SPOUT1) is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.

1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501

2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046

3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266

4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325

5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

Note

For research use only

图片

参考文献

产品说明书

Anti-Human SPOUT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

概述

图片

参考文献

产品说明书

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