ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
35.35 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
28S rRNA (uridine-N(3))-methyltransferase is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.
1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501
2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046
3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266
4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325
5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
