Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013)

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50ul
100ul

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货号	HC273013
品牌	abinScience
种属反应性	Human
应用	IF, FCM
宿主	Rabbit
克隆类型	Monoclonal
同种型	IgG
克隆号	SRR0102
经测试应用	IF, FCM
靶标	AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, CD69, CLEC2C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leukocyte surface antigen Leu-23, MLR-3
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q07108
状态	Liquid
保存溶液	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only

Immunofluorescence

Raji cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD69 (HC273013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD69 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD69 antibody. Permeabilized Transfected cells and Control cells were stained with an anti-Human CD69 monoclonal antibody (Catalog HC273013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD69 Recombinant Antibody (SRR0102).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013)

概述

图片

参考文献

产品说明书

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