Anti-Human PD-L1 Recombinant Antibody (SRR0417) (HV974133)

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50ul
100ul

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货号	HV974133
品牌	abinScience
种属反应性	Human
应用	IF, FCM, WB
宿主	Rabbit
克隆类型	Monoclonal
同种型	IgG
克隆号	SRR0417
经测试应用	IF, FCM, WB
靶标	B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, hPD-L1
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q9NZQ7
状态	Liquid
保存溶液	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only

Western blot

Western blot analysis was performed using anti-PD-L1 monoclonal antibody at 1 ug/mL on various samples.
Lane 1: PD-L1-overexpressing HEK293T cell lysate
Immunofluorescence

293T cells overexpressing PD-L1 were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting PD-L1 (HV974133) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of PD-L1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human PD-L1 antibody. Overexpression cells and Untransfected cells were stained with an anti-Human CD274 monoclonal antibody (Catalog HV974133, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human PD-L1 Recombinant Antibody (SRR0417).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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Anti-Human PD-L1 Recombinant Antibody (SRR0417) (HV974133)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PD-L1 Recombinant Antibody (SRR0417) (HV974133)

概述

图片

参考文献

产品说明书

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