Protein A/G purified from cell culture supernatant.
Accession号
Q9Z0J7
状态
Liquid
保存溶液
0.01M PBS pH 7.4.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
产品使用信息
应用方法
稀释比例
ELISA
1:2000-1:20000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Growth/differentiation factor 15 (GDF15/MIC1) is a ~34 kDa protein. Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea, vomiting, and/or loss of appetite. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues.
1. Tsai, VW. et al. (2013) PloS one 8, e55174. PMID: 23468844
2. Tsai, VW. et al. (2014) PloS one 9, e100370. PMID: 24971956
3. Mullican, SE. et al. (2017) Nature medicine 23, 1150-1157. PMID: 28846097
4. Emmerson, PJ. et al. (2017) Nature medicine 23, 1215-1219. PMID: 28846098
5. Yang, L. et al. (2017) Nature medicine 23, 1158-1166. PMID: 28846099
