| 货号 | ICH1212HU-25mg |
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| 品牌 | IchorBio |
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| 描述 |
Bulk Anti-DYKDDDDK Tag (M2-ICH) Antibody - Human IgG1 ( ICH1212HU-25mg) is a Human, clone Recombinant version of the M2 clone (Human IgG1) antibody targeting DYKDDDDK-tag. Validated for ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls..
Highlights
- ●Low Endotoxin — Suitable for in vivo and functional studies.
- ●High Purity — Minimal background for reliable results.
- ●Validated Applications — Tested in ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls..
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| 应用 | ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. |
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| 宿主 | Human |
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| 克隆类型 | Recombinant |
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| 同种型 | Human IgG1 |
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| 克隆号 | Recombinant version of the M2 clone |
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| 免疫原 | DYKDDDDK tag |
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| 经测试应用 | ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. |
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| 靶标 | DYKDDDDK-tag |
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| 特异性 | Alternative of the monoclonal FLAG M2 antibody for the detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only. |
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| 内毒素水平 | ≤ 1.0 EU/mg as determined by the LAL method |
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| 纯度 | >95% by SDS-PAGE |
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| 纯化方式 | The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A. |
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| 状态 | Liquid |
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| 保存溶液 |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 运输 | Blue ice |
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| 稳定性和存储 | This low endotoxin FLAG-tag antibody is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
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| 规格 | Size: 25mg |
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| 别名 | FLAG tag, M2 clone, DYKDDDDK peptide |
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| 背景 | Detecting and purifying DYKDDDDK (FLAG)-tagged proteins is a routine requirement in recombinant biology, and this human IgG1 anti-FLAG antibody, a recombinant version of the M2 clone, serves that role across immunoblotting, immunoprecipitation, ELISA, immunofluorescence, ChIP, flow cytometry, and tag-based protein purification. The M2-ICH reagent in human IgG1 format is supplied with low endotoxin for in vivo applications and manufactured in scalable milligram to gram quantities for bulk and high-throughput laboratories. As a research-use-only, non-therapeutic reagent, it offers reproducible lot-to-lot tag recognition for demanding experimental pipelines. |
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| Note | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
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| Isotype Control | ICH2254 |
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