| 背景 | This is a rat anti-mouse EpCAM (CD326) monoclonal antibody, clone G8.8, of the rat IgG2a isotype, produced in rat host. G8.8 is one of the most widely referenced anti-mouse EpCAM clones and recognises the mouse epithelial cell adhesion molecule expressed across epithelial tissues and on thymic epithelial cells. It is well suited to research applications that require reliable identification, gating, and enrichment of EpCAM-positive populations, and where an in-vivo-compatible preparation is preferred. Supplied as a low-endotoxin, RUO formulation (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and available in bulk milligram-to-gram quantities, the format supports flow cytometry, cell sorting, imaging, and studies that inject antibody into animals for tissue targeting, labelling, or receptor-engagement experiments. The rat IgG2a backbone provides a defined isotype for effector-function considerations and for pairing with appropriate isotype controls. As with all ichorbio in-vivo reagents, this product is intended for research use only and each lot should be validated by the investigator for the specific assay and model.
EpCAM (epithelial cell adhesion molecule; CD326, gene Epcam, UniProt Q99JW5) is a type I transmembrane glycoprotein expressed on the basolateral surface of most epithelia and on thymic epithelial cells. Despite its name, it functions less as a classical adhesion molecule and more as a signalling and homeostatic regulator: regulated intramembrane proteolysis releases its intracellular domain (EpICD), which can associate with beta-catenin and FHL2 to influence proliferation-associated transcription. EpCAM modulates cell-cell contacts, can antagonise E-cadherin-mediated adhesion, and contributes to epithelial proliferation, differentiation, and tissue morphogenesis. It is a defining marker of epithelial identity and is used to distinguish epithelial from mesenchymal and haematopoietic cells. EpCAM is frequently overexpressed on carcinomas and is a marker of circulating tumour cells, making it a heavily studied epithelial and oncology antigen. In mouse models it is a standard readout for epithelial, tumour-epithelial, and thymic epithelial populations. |
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