Bulk anti-Human CD19 (4G7) Antibody (ICH1237-1mg)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This is a bulk-format, research-grade monoclonal antibody (clone 4G7, mouse IgG1 kappa) raised against human CD19. Clone 4G7 is a widely referenced anti-human CD19 antibody used across flow cytometry, immunofluorescence, functional B-cell assays, and chimeric antigen receptor (CAR) research where CD19 serves as the archetypal target antigen. Produced as a mouse host antibody with defined isotype, it is suited to functional and cell-based work requiring consistent lot performance and larger quantities. The antibody is offered at low endotoxin levels (research grade <1 EU/mg), supporting sensitive primary-cell and functional assays where endotoxin contamination can confound results. Bulk milligram-to-gram scale supports high-throughput screening, repeated assays, reagent standardization, and CAR-T or CAR-construct development pipelines that consume larger amounts of reference reagent. All material is supplied for research use only (RUO). Because the target is human CD19, this reagent is intended for in-vitro human-cell applications rather than mouse in-vivo models; investigators should validate performance in their own system. CD19 is a 95 kDa transmembrane glycoprotein of the immunoglobulin superfamily expressed throughout most of B-cell development, from pro-B/pre-B stages through mature B cells, but lost upon terminal plasma-cell differentiation. It functions as a positive co-receptor that lowers the threshold for B-cell receptor (BCR) signaling, associating with CD21, CD81, and CD225 in the B-cell co-receptor complex. Upon engagement, its cytoplasmic tyrosine residues are phosphorylated and recruit PI3K and other effectors, amplifying BCR-driven activation, proliferation, and survival. Because its expression is highly restricted to the B lineage and is retained on most B-cell malignancies, CD19 is a leading immunotherapy target, underpinning CD19-directed CAR-T cells and bispecific antibodies. Its consistent lineage expression also makes it a core marker for identifying and gating B cells in immunophenotyping.