Anti-RB1 Polyclonal Antibody (HY189014)

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概述
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参考文献
产品说明书

概述

货号

HY189014

品牌

abinScience

描述

Anti-RB1 Polyclonal Antibody (HY189014) is a rabbit polyclonal antibody detecting RB1 in ELISA, IHC, WB. Suitable for Mouse, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human RB1 (Gln639-Thr778).

靶标

pRb, p110-RB1, Retinoblastoma-associated protein, Rb, p105-Rb, RB1, pp110

纯化方式

Purified by antigen affinity column.

Accession号

P06400

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Retinoblastoma-associated protein (RB1) is a ~106 kDa protein. Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C.

1. Harbour, JW. et al. (1999) Cell 98, 859-69. PMID: 10499802

2. Ren, S. et al. (2004) Cell 117, 239-51. PMID: 15084261

Note

For research use only.

图片

参考文献

产品说明书

Anti-RB1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RB1 Polyclonal Antibody (HY189014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RB1 Polyclonal Antibody (HY189014)

概述

图片

参考文献

产品说明书

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