Anti-CDK4 Polyclonal Antibody (HY215014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

HY215014

品牌

abinScience

描述

Anti-CDK4 Polyclonal Antibody (HY215014) is a rabbit polyclonal antibody detecting CDK4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Ovis aries.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human CDK4 (Met1-Glu303).

靶标

Cell division protein kinase 4, PSK-J3, Cyclin-dependent kinase 4, CDK4

纯化方式

Purified by antigen affinity column.

Accession号

P11802

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Cyclin-dependent kinase 4 (CDK4) is a ~33 kDa protein. Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity.

文献

Circular RNA CLASP1 modulates the GLI1/SNAIL axis and enhances macrophage polarization in breast cancer.[HY215014]

Note

For research use only.

图片

Western blot

Western blot analysis of CCND1 and CDK4 protein levels in MDA-MB-231 and MCF-7 cells transfected with sh-circCLASP1 or sh-scramble.

参考文献

Circular RNA CLASP1 modulates the GLI1/SNAIL axis and enhances macrophage polarization in breast cancer

产品说明书

Anti-CDK4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CDK4 Polyclonal Antibody (HY215014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CDK4 Polyclonal Antibody (HY215014)

概述

图片

参考文献

产品说明书

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