Anti-MIF Polyclonal Antibody (HB816014)

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概述
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产品说明书

概述

货号

HB816014

品牌

abinScience

描述

Anti-MIF Polyclonal Antibody (HB816014) is a rabbit polyclonal antibody detecting MIF in ELISA, IHC, WB. Suitable for Bovine, Human, Macaca fascicularis, Macaca mulatta, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MIF (Met1-Ala115).

靶标

MIF, L-dopachrome isomerase, L-dopachrome tautomerase, GIF, Macrophage migration inhibitory factor, MMIF, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF

纯化方式

Purified by antigen affinity column.

Accession号

P14174

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Macrophage migration inhibitory factor (MIF/GIF) is a ~12 kDa protein. Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

1. Oddo, M. et al. (2005) Infection and immunity 73, 3783-6. PMID: 15908412

2. Emonts, M. et al. (2007) Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 44, 1321-8. PMID: 17443469

3. Fan, C. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10994-9. PMID: 23776208

4. Tan, TH. et al. (2001) The Biochemical journal 357, 373-83. PMID: 11439086

Note

For research use only.

图片

参考文献

产品说明书

Anti-MIF Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MIF Polyclonal Antibody (HB816014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MIF Polyclonal Antibody (HB816014)

概述

图片

参考文献

产品说明书

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