Anti-WT1 Polyclonal Antibody (HB628014)

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概述
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产品说明书

概述

货号

HB628014

品牌

abinScience

描述

Anti-WT1 Polyclonal Antibody (HB628014) is a rabbit polyclonal antibody detecting WT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pig, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human WT1 (Thr317-Gln446).

靶标

WT1, Wilms tumor protein, WT33

纯化方式

Purified by antigen affinity column.

Accession号

P19544

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Wilms tumor protein (WT1) is a ~49 kDa protein. Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

1. Hamilton, TB. et al. (1995) Nucleic acids research 23, 277-84. PMID: 7862533

2. Stoll, R. et al. (2007) Journal of molecular biology 372, 1227-45. PMID: 17716689

3. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363

4. Smolen, GA. et al. (2004) Cancer research 64, 7846-51. PMID: 15520190

5. Markus, MA. et al. (2006) Experimental cell research 312, 3379-88. PMID: 16934801

6. Weiss, TC. et al. (2009) Biochemistry 48, 148-55. PMID: 19123921

Note

For research use only.

图片

参考文献

产品说明书

Anti-WT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-WT1 Polyclonal Antibody (HB628014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-WT1 Polyclonal Antibody (HB628014)

概述

图片

参考文献

产品说明书

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