Anti-ACAT1 Polyclonal Antibody (HB220014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB220014

品牌

abinScience

描述

Anti-ACAT1 Polyclonal Antibody (HB220014) is a rabbit polyclonal antibody detecting ACAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ACAT1 (Ala111-Leu427).

靶标

ACAT, Acetoacetyl-CoA thiolase, MAT, Acetyl-CoA acetyltransferase, mitochondrial, ACAT1, T2

纯化方式

Purified by antigen affinity column.

Accession号

P24752

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a ~45 kDa protein. This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.

1. Fukao, T. et al. (1991) Biochemical and biophysical research communications 179, 124-9. PMID: 1715688

2. Wakazono, A. et al. (1995) Human mutation 5, 34-42. PMID: 7728148

3. Fukao, T. et al. (1998) Human mutation 12, 245-54. PMID: 9744475

4. Haapalainen, AM. et al. (2007) Biochemistry 46, 4305-21. PMID: 17371050

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ACAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Caco2 cell lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse liver lysate
Lane 4: Rat heart lysate
Lane 5: Rat skeletal muscle lysate
Lane 6: Rat liver lysate

参考文献

产品说明书

Anti-ACAT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ACAT1 Polyclonal Antibody (HB220014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ACAT1 Polyclonal Antibody (HB220014)

概述

图片

参考文献

产品说明书

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