Anti-NFKBIA Polyclonal Antibody (HB155014) is a rabbit polyclonal antibody detecting NFKBIA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human NFKBIA (Lys67-Glu302).
靶标
I-kappa-B-alpha, IKBA, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法
稀释比例
ELISA
1:2000-1:20000
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
NF-kappa-B inhibitor alpha (NFKBIA) is a ~35 kDa protein. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
1. Ganchi, PA. et al. (1992) Molecular biology of the cell 3, 1339-52. PMID: 1493333
2. Quan, J. et al. (2023) Journal of immunology (Baltimore, Md. : 1950) 210, 640-652. PMID: 36651806
3. Scherer, DC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11259-63. PMID: 7479976
4. Chen, Z. et al. (1995) Genes & development 9, 1586-97. PMID: 7628694
5. Traenckner, EB. et al. (1995) The EMBO journal 14, 2876-83. PMID: 7796813
6. Brown, K. et al. (1995) Science (New York, N.Y.) 267, 1485-8. PMID: 7878466
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-NFKBIA polyclonal antibody at 1ug/mL on various samples. Lane 1: NIH3T3 cell lysate
