Anti-CDC25B Polyclonal Antibody (HB248014)

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概述
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参考文献
产品说明书

概述

货号

HB248014

品牌

abinScience

描述

Anti-CDC25B Polyclonal Antibody (HB248014) is a rabbit polyclonal antibody detecting CDC25B in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human CDC25B (Cys377-Gly566).

靶标

Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2, M-phase inducer phosphatase 2

纯化方式

Purified by antigen affinity column.

Accession号

P30305

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

M-phase inducer phosphatase 2 (CDC25B) is a ~64 kDa protein. Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. The three isoforms seem to have a different level of activity.

1. Galaktionov, K. et al. (1991) Cell 67, 1181-94. PMID: 1836978

2. Timofeev, O. et al. (2010) The Journal of biological chemistry 285, 16978-90. PMID: 20360007

3. Schmidt, A. et al. (2007) The EMBO journal 26, 1624-36. PMID: 17332740

Note

For research use only.

图片

参考文献

产品说明书

Anti-CDC25B Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CDC25B Polyclonal Antibody (HB248014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CDC25B Polyclonal Antibody (HB248014)

概述

图片

参考文献

产品说明书

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