Anti-BMI1 Polyclonal Antibody (HW616014)

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概述
图片
参考文献
产品说明书

概述

货号

HW616014

品牌

abinScience

描述

Anti-BMI1 Polyclonal Antibody (HW616014) is a rabbit polyclonal antibody detecting BMI1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Cat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human BMI1 (Glu152-Ile289).

靶标

RING finger protein 51, BMI1, RNF51, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, PCGF4

纯化方式

Purified by antigen affinity column.

Accession号

P35226

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Polycomb complex protein BMI-1 (BMI1) is a ~36 kDa protein. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.

1. Wang, H. et al. (2004) Nature 431, 873-8. PMID: 15386022

2. Cao, R. et al. (2005) Molecular cell 20, 845-54. PMID: 16359901

3. Li, Z. et al. (2006) The Journal of biological chemistry 281, 20643-9. PMID: 16714294

4. Bentley, ML. et al. (2011) The EMBO journal 30, 3285-97. PMID: 21772249

5. McGinty, RK. et al. (2014) Nature 514, 591-6. PMID: 25355358

6. Taherbhoy, AM. et al. (2015) Nature communications 6, 7621. PMID: 26151332

7. Gray, F. et al. (2016) Nature communications 7, 13343. PMID: 27827373

Note

For research use only.

图片

参考文献

产品说明书

Anti-BMI1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BMI1 Polyclonal Antibody (HW616014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BMI1 Polyclonal Antibody (HW616014)

概述

图片

参考文献

产品说明书

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