Anti-MDH1 Polyclonal Antibody (HW769014)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

HW769014

品牌

abinScience

描述

Anti-MDH1 Polyclonal Antibody (HW769014) is a rabbit polyclonal antibody detecting MDH1 in ELISA, IHC, WB. Suitable for Bovine, Cat, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human MDH1 (Ser2-Ala334).

靶标

MDHA, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, MDH1, Diiodophenylpyruvate reductase

纯化方式

Purified by antigen affinity column.

Accession号

P40925

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Malate dehydrogenase, cytoplasmic (MDH1) is a ~36 kDa protein. Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS).

1. Friedrich, CA. et al. (1987) Biochemical genetics 25, 657-69. PMID: 2449162

2. Friedrich, CA. et al. (1988) Annals of human genetics 52, 25-37. PMID: 3052244

3. Broeks, MH. et al. (2019) Human genetics 138, 1247-1257. PMID: 31538237

4. Zhang, JY. et al. (2021) Cell research 31, 980-997. PMID: 34012073

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-MDH1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: THP-1 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate
Lane11: Rat liver lysate
Lane12: Rat kidney lysate

参考文献

产品说明书

Anti-MDH1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MDH1 Polyclonal Antibody (HW769014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MDH1 Polyclonal Antibody (HW769014)

概述

图片

参考文献

产品说明书

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