Anti-CASP4 Polyclonal Antibody (HW474014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HW474014

品牌

abinScience

描述

Anti-CASP4 Polyclonal Antibody (HW474014) is a rabbit polyclonal antibody detecting CASP4 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human CASP4 (Asn78-Glu265).

靶标

Mih1, ICH2, CASP-4, ICE and Ced-3 homolog 2, CASP4, Protease TX, Caspase-4, ICE(rel)-II, ICH-2

纯化方式

Purified by antigen affinity column.

Accession号

P49662

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Caspase-4 (CASP4) is a ~43 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B. In the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without the need of an upstream sensor.

1. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034

2. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003

3. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412

4. Li, Z. et al. (2021) Nature 599, 290-295. PMID: 34671164

5. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519

6. Devant, P. et al. (2023) Nature 624, 451-459. PMID: 37993712

7. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

8. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

10. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478

Note

For research use only.

图片

参考文献

产品说明书

Anti-CASP4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CASP4 Polyclonal Antibody (HW474014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CASP4 Polyclonal Antibody (HW474014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价