Anti-WNT3A Polyclonal Antibody (HX182014)

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概述
图片
参考文献
产品说明书

概述

货号

HX182014

品牌

abinScience

描述

Anti-WNT3A Polyclonal Antibody (HX182014) is a rabbit polyclonal antibody detecting WNT3A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Chicken, and Meleagris gallopavo.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human WNT3A (Ser19-Lys352).

靶标

Protein Wnt-3a, WNT3A

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P56704

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein Wnt-3a (WNT3A) is a ~39 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).

1. Bourhis, E. et al. (2010) The Journal of biological chemistry 285, 9172-9. PMID: 20093360

2. Doubravska, L. et al. (2011) Cellular signalling 23, 837-48. PMID: 21244856

3. MacDonald, BT. et al. (2014) The Journal of biological chemistry 289, 18122-36. PMID: 24841207

4. Mihara, E. et al. (2016) eLife 5. PMID: 26902720

Note

For research use only.

图片

参考文献

产品说明书

Anti-WNT3A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-WNT3A Polyclonal Antibody (HX182014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-WNT3A Polyclonal Antibody (HX182014)

概述

图片

参考文献

产品说明书

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