Anti-Human CD10/MME Recombinant Antibody (NL-1) (HY401107)

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100ug

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货号	HY401107
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG1, kappa
克隆号	NL-1
靶标	Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P08473
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). 1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675 2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535 3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683 4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535 5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991 6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193
Note	For research use only.

SEC-HPLC

SEC-HPLC detection for Anti-Human CD10/MME Antibody (NL-1).
Bioactivity

Detects CD10/MME in indirect ELISAs.
Flow CytoMetry

Flow-cytometry using anti-human CD10 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD10 monoclonal antibody (Catalog: HY401107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD10/MME Recombinant Antibody (NL-1).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

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Anti-Human CD10/MME Recombinant Antibody (NL-1) (HY401107)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD10/MME Recombinant Antibody (NL-1) (HY401107)

概述

图片

参考文献

产品说明书

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