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Anti-Human CD10/MME Recombinant Antibody (NL-1), FITC (HY401117)

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规格:
  • 50T
  • 100T
数量:
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概述
货号HY401117
品牌abinScience
种属反应性Human
应用FCM
宿主Mouse
同种型IgG1, kappa
克隆号NL-1
偶联物FITC
克隆类型Monoclonal
靶标Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN
内毒素水平Please contact with the lab for this information.
相关产品 Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
Accession号P08473
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)).

1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675
2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535
3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683
4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535
5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991
6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow Cytometry

    Flow-cytometry using FITC anti-human CD10/MME antibody. Daudi cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-human CD10/MME antibody monoclonal antibody (Catalog # HY401117, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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