Anti-Human CD2 Recombinant Antibody (G11) (HY113107)

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100ug

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货号	HY113107
品牌	abinScience
种属反应性	Human
应用	FCM
同种型	IgG2a, kappa
克隆号	G11
经测试应用	WB, FCM
靶标	Erythrocyte receptor, SRBC, T-cell surface antigen CD2, Rosette receptor, CD2, LFA-3 receptor, T-cell surface antigen T11/Leu-5, LFA-2
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P06729
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	T-cell surface antigen CD2 (LFA-2/OX-34) is a ~39 kDa protein. CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Note	For research use only.

Bioactivity

Detects CD2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD2 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD2 antibody monoclonal antibody (Catalog # HY113107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD2 (Catalog No: HY113011)
Lane 2: negative control

产品说明书

Anti-Human CD2 Recombinant Antibody (G11).pdf

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Anti-Human CD2 Recombinant Antibody (G11) (HY113107)

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Anti-Human CD2 Recombinant Antibody (G11) (HY113107)

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