Human CD2 Recombinant Protein (C-His) (HY113011)

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100ug
1mg

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概述
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参考文献
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概述

货号	HY113011
品牌	abinScience
描述	Recombinant Human CD2 Protein, C-His (HY113011) expressed in Mammalian Cells, spanning Met1-Asp209. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	Mammalian cells
Accession号	P06729
蛋白长度	Met1-Asp209
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
经测试应用	WB
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
预测分子量	24.78 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	Erythrocyte receptor, SRBC, T-cell surface antigen CD2, Rosette receptor, CD2, LFA-3 receptor, T-cell surface antigen T11/Leu-5, LFA-2
背景	T-cell surface antigen CD2 (LFA-2/OX-34) is a ~39 kDa protein. CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CD2 Protein, C-His
Bioactivity

Detects Siplizumab in indirect ELISAs.
Bioactivity

Detects Alefacept in indirect ELISAs.
Bioactivity

Detects Human CD2 Antibody (G11) in indirect ELISAs.
Western blot

Detected by Anti-CD2 monoclonal antibody (Catalog No: HY113107)
Western blot

Detected by Anti-CD2 monoclonal antibody (Catalog No: HY113207)
Bioactivity

Immobilized HY113011 Human CD2 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HB078021 Human CD58 Recombinant Protein (C-Fc), the EC 50 is 3.38 μg/mL.
Bioactivity

Immobilized HB078021 Human CD58 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HY113011 Human CD2 Recombinant Protein (C-His), the EC 50 is 1.24 μg/mL.

参考文献

产品说明书

Human CD2 Recombinant Protein (C-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Human CD2 Recombinant Protein (C-His) (HY113011)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Human CD2 Recombinant Protein (C-His) (HY113011)

概述

图片

参考文献

产品说明书

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